Skip to main content
. 2020 Jun 25;11(6):486. doi: 10.1038/s41419-020-2689-4

Fig. 5. lncRNA ENST00000563492 promotes angiogenesis in HUVECs.

Fig. 5

a Scratch assays were used to detect the migratory ability of HUVECs, and photographs were taken at 0 and 24 h after adding the conditioned medium from BMSCs transfected with Lenti-ENST00000563492, pLKO.1-ENST00000563492, or control plasmid. b Transwell assays were used to detect the invasion ability of HUVECs, and photographs were taken at 24 h after adding the conditioned medium from BMSCs transfected with Lenti-ENST00000563492 or pLKO.1-ENST00000563492 or control plasmid in the lower chamber. c Tube-formation analysis of angiogenesis in HUVECs and d quantitative analysis. e ELISA analysis of the secretion of VEGF, endostatin, PAI-1, and thrombospondin-1 in culture medium of BMSCs transfected with Lenti-ENST00000563492, pLKO.1-ENST00000563492, or control plasmid. BMSCs isolated from patient no. 1, 2, 5, 12, 10, 14, 19, 20, 21, 22, 23, and 24. All experiments were performed at least three times. Scale bar = 100 μm. The results are presented as mean ± SD. *p < 0.05, **p < 0.01.