Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 19;11:1252. doi: 10.3389/fmicb.2020.01252

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Li, Zhu, Zhang, Lin, You, Chen, Wang, Zhu and Si.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Construction of the Y2H system to detect BamA–BamD interaction. (A) The strategy for high-throughput screening using the Y2H system. BamA–BamD interaction induces the expression of reporter genes, ADE2, HIS3, and LacZ. The compounds that disrupt BamA–BamD interaction prevent the expression of these reporter genes. (B) The growth and LacZ-dependent color change of yeast cells with various combinations of BD and AD fusions. The left panel shows the growth of yeast cells with indicated plasmids on an SD/-Leu-Trp-Ade-His dropout plate. The right panel shows the β-gal activity of indicated strains in SD/-Leu-Trp dropout plate. (C) Quantification of β-gal activity in yeast cells. The results are the average from triplicated assays. (D) The expression of BamA and BamD proteins in yeast cells. The expression was detected using anti-HA and anti-Myc antibodies.