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. 2020 Jun 19;11:1252. doi: 10.3389/fmicb.2020.01252

FIGURE 3.

FIGURE 3

Identification of potential compound that disrupts BamA–BamD interaction. (A) Growth inhibition of yeast cells by IMB-H4. Yeast strains with indicated plasmids seeded in 96-well plates were incubated in SD/-Leu-Trp-Ade-His dropout medium with duplicates. The final concentration of IMB-H4 was from 5 to 100 μg/mL. (B) The inhibition of β-gal activity of AH109 (pAD-BamA + pBD-BamD) cells by IMB-H4 at multiple concentrations. Strain AH109 (pAD-T + pBD-53) was applied as a control. Values represent the ratio of β-gal activity of cells treated with compounds over that of untreated cells. The results are the average units from triplicated assays. (C) Structure of compound IMB-H4.