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. 2020 Jun 19;11:1072. doi: 10.3389/fimmu.2020.01072

Figure 4.

Figure 4

shRNA-mediated knockdown and rescue of TRAIL influence HTNV replication and apoptosis in HUVECs. HUVECTRAIL−KD cells were infected with HTNV76-118 (MOI = 1) for 2 h, treated with/without rTRAIL (40 ng/mL), and then followed by qRT-PCR and Western blot at different days to measure TRAIL-related apoptosis factors and HTNV. The mRNA results shown were the average of three replicates. Values represent the mean ± SD (*p < 0.05; **p < 0.01; ***, p 0.001). (A) HTNV S mRNA, (B) TRAIL mRNA, (C) DR4 mRNA, (D) DR5 mRNA, (E) DcR1 mRNA, (F) DcR2 mRNA, (G) caspase-8 mRNA, and (H) caspase-3 mRNA. (I) HTNV NP and TRAIL-related apoptosis proteins in HTNV-infected HUVECTRAIL−KD cells at 2, 3, and 4 dpi. (J) HTNV NP and TRAIL-related apoptosis proteins of HUVECTRAIL−KD cells with rTRAIL rescue. Proteins were quantified using carestream software and compared with the mock group of 2 dpi; the data shown represents one of three similar independent experiments. The numbers represent the relative density of the bands relative to the corresponding control.