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. 2020 Jun 19;11:690. doi: 10.3389/fphys.2020.00690

FIGURE 2.

FIGURE 2

11-week-old, mdx male muscles display altered regulators of the mitochondrial life cycle, enzymatic activity, and mitochondrial shape. (A) Mitochondrial DNA copy number in quadriceps muscle (N = 6–8). (B) mRNA expression in quadriceps muscle (N = 5–8). (C) Protein expression in quadriceps muscle (N = 6–8). (D) Protein expression in mitochondria isolated from quadriceps muscle (N = 6–8). (E) Electron micrograph images of the soleus muscle with quantified mitochondrial area and cristae numbers per area of mitochondria shown right. (F) HE, SDH, and COX staining in tibialis anterior muscle with the percentage of muscle fiber density shown right (N = 13). Black arrow indicates fibers with centralized nuclei. Only some fibers are indicated. Scale bar = 0.1 mm. Data presented as Mean ± SEM. *, **, ***P < 0.05, 0.01, 0.001, respectively.