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. 2020 Jun 19;11:690. doi: 10.3389/fphys.2020.00690

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Copyright © 2020 Moore, Lin, Strumwasser, Cory, Whitney, Ho, Ho, Lee, Rucker, Nguyen, Yackly, Mahata, Wanagat, Stiles, Turcotte, Crosbie and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mitochondria from 2-week-old, mdx male muscles display altered size, cristae numbers per area of mitochondria, and enzymatic activity. (A) Mitochondrial DNA copy number in quadriceps muscle (N = 5). (B) Immunoblot images from quadriceps muscle (N = 6–8, showing N = 3). (C) HE stain of gastrocnemius muscle. Scale bar = 0.1 mm. (D) Electron micrograph images of the soleus muscle with quantified mitochondrial area and cristae numbers per area of mitochondria shown right. Black arrows indicate aberrant mitochondria. (E) Complex IV activity in quadriceps muscle (N = 5). Data presented as Mean ± SEM. (F) Mitochondrial respirometry analysis in frozen quadriceps muscle (N = 6–8). *P < 0.05.