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. 2020 Jun 19;11:584. doi: 10.3389/fphys.2020.00584

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Copyright © 2020 Mahmutovic Persson, Falk Håkansson, Örbom, Liu, von Wachenfeldt and Olsson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Study layout and imaging workflow chart. (A) Study layout of the DIILD model with bleomycin-induced lung injury in Sprague–Dawley male rats. Imaging was performed at baseline (scan 1) before the intratracheal (i.t.) administration of bleomycin. Control rats received saline as i.t. challenge. Imaging sessions occurred at day 0, 3, 7, 14, 21, and 28 post challenge. Before every scan session, blood glucose was measured. Termination occurred at day 28, after the final imaging session. (B) Workflow for each imaging session. Events expressed in gray (35 and 50 min after injection) indicate when the following rat is prepared while the scan of the previous rat is being performed. i.v. = intravenous.