FIG 5.

(A) Membrane fluidity in the wild type, fakA mutant, crtM mutant, and fakA crtM mutant grown in the presence (+OA) or absence (TSB) of 0.01% oleic acid. (B) Membrane integrity measured using propidium iodide (PI) as an indicator for the wild type (black) and fakA mutant (red). (C) Membrane potential was analyzed using DiOC(3)₂ staining of the wild type, fakA mutant, and complemented fakA mutant (comp). (D) Respiratory activity of the wild type, fakA mutant, and complemented fakA mutant as measured using CTC as an indicator. Data represent the means (n = 3) with SD. *, P < 0.05 by Student’s t test. “#” indicates that all samples treated with OA differed significantly from the corresponding strain in TSB alone. Data are representative of those from at least three independent experiments.