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. 2019 Sep 10;2(Suppl 1):i75–i84. doi: 10.1093/noajnl/vdz026

Figure 4.

Figure 4.

Loss of Trim23 leads to intrinsic tumor biology changes. (A) Relative mRNA expression of Trim23 in Nf1/Tp53-mutant NPcis murine MPNST cell lines transduced with shLacZ control or 3-unpooled shTrim23 viral particles. (B) Capillary Western blot of aforementioned cells, blotting for Trim23 with quantification. (C) An Incucyte cell proliferation assay measuring cell confluence over time. (D) Colony formation assay quantifying percent confluence. (E) Representative images of subcutaneous injected mice tumor burden at 16 days postinjection. (F) Quantification of BLI (photon flux) for subcutaneous injection models (n = 5 per group). (G) Representative images of left ventricle injected mice tumor burden at 16 days postinjection (n = 5 per group). (H) Quantification of BLI (photon flux) for left ventricle injection models (n = 5, * P < .05, ** P < .001, *** P < .0001).