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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Curr Protoc Neurosci. 2020 Jun;92(1):e93. doi: 10.1002/cpns.93

Figure 4. Optimizing the conditions for Ribotag assay based on ribosomal integrity.

Figure 4.

Bioanalyzer traces, measuring the RNA Integrity (RIN) Values of RNA obtained from the bulk hippocampal tissue (Input/total RNA fraction), in Green box, left; vs. hippocampal MGE interneuron-specific RNA (Rpl22-HA immunoprecipitation fraction), in Purple box, right. Comparing quality of RNA in Nkx2.1 CrePositive RibotagHeterozygous animals between (A) tissue processed after flash-freezing in liquid nitrogen Vs, (B) tissue processed from fresh animals. Comparing quality of RNA between tissue processed fresh in Nkx2.1 CrePositive (B) RibotagHeterozygous animals Vs, (C) RibotagHomozygouss animals. (D) Negative control for the Ribotag assay using Nkx2.1 CreNegative RibotagHomozygouss animals. Abbreviations: RIN, RNA Integrity; IP, immunoprecipitation; Ribo, Ribotag; MGE, Medial ganglionic eminence.