| 1 cycle | 94°C | 2 min |
| 10 cycles | 94°C | 20sec |
| 65°C (−1.5°C per cycle) | 15sec | |
| 68°C | 10sec | |
| 28 cycles | 94°C | 15sec |
| 50°C | 15sec | |
| 72°C | 10sec | |
| 1 cycle | 72°C | 2min |
| 10°C | hold |
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