| Stock | Working | For 10ml |
|---|---|---|
| 1M Tris HCl (pH7.5) | 50mM | 500μl |
| 3M KCl | 300mM | 1ml |
| 1M MgCl2 | 12mM | 120μl |
| 20% (v/v) NP-40 SurfactAmps | 1% | 500μl |
| 100mM DTT | 1mM | 100μl |
| 100mg/ml Cyclohexamide | 100μg/ml | 100μl |
| 40U/μl RNAseIN | 400U/ml | 100μl |
| 10mg/ml Heparin | 1mg/ml | 20mg |
| Complete mini Protease inhibitor | - | 1 tablet |
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