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Standards for Protein Estimation by Bicinchoninic Acid (BCA) Assay;
| Concentration μg/ml | Ultrapure water (in μl) | Standard BSA 1mg/ml (in μl) |
|---|---|---|
| Blank | 25 | 0 |
| 100 | 22.5 | 2.5 |
| 200 | 20 | 5 |
| 300 | 17.5 | 7.5 |
| 400 | 15 | 10 |
| 500 | 12.5 | 12.5 |
| 600 | 10 | 15 |
| 700 | 7.5 | 17.5 |
| 800 | 5 | 20 |
| 900 | 2.5 | 22.5 |
| 1000 | 0 | 25 |
