Table 2.
List of Suggested Primers for the qRT-PCR Assays for Use in Nkx2.1-Cre Mice
| Marker | GenBank no. | Sense primers | Antisense primers | Size (bp) |
|---|---|---|---|---|
| Gad1 | NM_008077.4 | ATGATACTTGGTGTGGCGTAGC | GTTTGCTCCTCCCCGTTCTTAG | 253 |
| Gad2 | NM_008078.2 | CCAAAAGTTCACGGGCGG | TCCTCCAGATTTTGCGGTTG | 375 |
| Lhx6 | NM_008500.2 | TGCCATGGCTCAGTCAGACGCA | CCTTCTCCTCAACGAGGCCGAATT | 473 |
| Pvalb | NM_013645.3 | GCCTGAAGAAAAAGAACCCG | AATCTTGCCGTCCCCATCCT | 275 |
| Sst | NM_009215.1 | ATGCTGTCCTGCCGTCTCCA | GCCTCATCTCGTCCTGCTCA | 250 |
| Vip | NM_011702.2 | TTATGATGTGTCAAGAAATGCCAG | TTTTATTTGGTTTTGCTATGGAAG | 424 |
| Slc17a7 | NM_182993.2 | CCCTTAGAACGGAGTCGGCT | TATCCGACCACCAGCAGCAG | 593 |
| GFAP | NM_010277.3 | AAGCCACCCTGGCTCGTGTG | CTGTTCGCGCATTTGCCGCT | 407 |
| Tubb3, b3tuj1 | NM_023279.2 | CTTTTCGTCTCTAGCCGCGT | CCACCCAGTGAGTGTGTCAG | 451 |
| Actb | NM_007393.5 | CCTGACCGAGCGTGGCTACAGCTTC | CTGCTGGAAGGTGGACAGTGAGGCC | 487 |