Fig. 5. Identification of chemical constituents of 70% ethanolic extract of Gardenia jasminoides (GJEtOH) using high performance liquid chromatography (HPLC).
(A, B) HPLC chromatograms of standard chemicals (A), including geniposidic acid, chlorogenic acid, geniposide, genipin, and rutin, and HPLC chromatograms of GJEtOH (B). The detection of chemical constituents in GJEtOH was performed by comparing the retention times of the UV spectral peak with those of the standard chemicals at 238 nm. (C, D) HPLC chromatograms of standard chemicals (C), including crocin and HPLC chromatograms of GJEtOH (D) detected at 440 nm. (E, F) HPLC chromatograms of standard chemicals (E), including crocin II and HPLC chromatograms of GJEtOH (F) detected at 440 nm.