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. 2020 Jul 1;24(4):363–372. doi: 10.4196/kjpp.2020.24.4.363

Fig. 8. Genipin inhibits the proliferation of CD3/CD28 co-stimulated primary human CD4+ T lymphocytes.

Fig. 8

(A) A representative plot shows division of human naïve CD4+ T cells as assessed using carboxyfluorescein succinimidyl ester (CFSE) dilution. Human naïve CD4+ T cells were stained with 1 μM CFSE, treated with 10, 30, and 100 μM genipin (GnP), and cultured in anti-CD3 hAb-coated plates with 2 μg/ml anti-CD28. Cell proliferation was assessed by flow cytometry after 72 h of CD3/CD28 receptor stimulation. Human naïve CD4+ T cells stimulated with anti-CD3 and anti-CD28 only were used as negative controls; those treated with 10 μM BTP2 and stimulated with anti-CD3 and anti-CD28, served as positive control. (B) Statistical analysis summarizing the results of three independent experiments and indicating the relative proportion of CD4+ T cells to the whole T cell population induced by CD3/CD28 co-stimulation. *p < 0.05, ****p < 0.0001 vs. the control.