Fig. 1. Ethanolic (30%) extract of Spirodela polyrhiza (SPEtOH) inhibits anoctamin-1 (ANO1)-mediated Cl– current (IANO1) in ANO1-overexpressing HEK293T (HEKTANO1) cells.
(A) Representative chart trace recordings showing the effects of 30, 100, and 300 μg/ml SPEtOH on IANO1. At 300 μg/ml, SPEtOH almost fully inhibited IANO1, similar to the effects of 30 μM T16Ainh-A01 (AO-1). (B) Typical current (I)/voltage (V) relationship curves for IANO1, showing the inhibition of IANO1 by 30, 100, and 300 μg/ml SPEtOH. (C) Summary of IANO1 inhibition at +100 mV. Normalized amplitudes of currents (IExt/Ictrl × 100%) were measured at a clamp voltage of +100 mV. Data are presented as the mean ± standard error of mean. Ext, SPEtOH; ctrl, control. ****p < 0.0001 compared to the control (n = 6).