Figure 6.

Indirect effect of RBP4 on human SVF‐derived adipocytes (A). Media from RAW macrophages incubated with RBP4 (RBP4 CM) increases basal (−) and stimulated (+) lipolysis in human adipocytes. *P < .05 versus (−) same condition, #P < .05 versus Basal same media condition. (B) Insulin inhibition of RBP4 CM‐induced lipolysis has an ED50 over 30 pM compared to 10 pM for insulin inhibition of isoproterenol‐induced lipolysis (data not shown). Control CM: media from RAW cells incubated with vehicle only without RBP4. No CM: adipocytes incubated without any conditioned media. **P < .05 versus all other conditions. (C) Concentration of TNFα in media of RAW cells incubated without (open bar) or with RBP4 (50 µg/mL) (black bar). *P < .05 versus conditioned media without RBP4