Table 4.
The results of virologic and serologic testing of in‐contact horse sera housed at the same stables of the first equine WNND case in Austria in 2016
| Horse no. |
RT‐qPCR WNV |
IgMa | IgGa |
PRNT80b WNV |
PRNT80b TBEV |
PRNT80b USUV |
|---|---|---|---|---|---|---|
| 1 | − | − | − | nt | nt | nt |
| 2 | − | − | − | nt | nt | nt |
| 3 | − | − | − | nt | nt | nt |
| 4 | − | − | +/− | − | nt | nt |
| 5 | − | − | − | nt | nt | nt |
| 6 | − | − | +/− | − | nt | nt |
| 7 | − | − | +/− | − | nt | nt |
| 8 | − | − | + | − | nt | nt |
| 9 | − | − | + | 1:80 | − | − |
| 10 | − | − | − | nt | nt | nt |
| 11 | − | − | − | nt | nt | nt |
| 12 | − | − | − | nt | nt | nt |
| 13 | − | − | + | 1:160 | − | − |
| 14 | − | − | + | − | nt | nt |
+, positive; −, negative; +/−, weak positive; nt, not tested.
a
Commercial ELISA kits (IDVet) were used to measure the presence of IgG and IgM antibodies reactive to West Nile virus (WNV) antigen.
b
Endpoint titres for 80% plaque reduction neutralization assay (PRNT80) were performed for antibody‐positive samples against WNV, tick‐borne encephalitis virus (TBEV) and Usutu virus (USUV).