Table 4.
Effect of the nitrogen content, supplemented to the cultivation media in the form of monosodium glutamate (MSG) and ammonium chloride (NH4Cl), on the biomass and polymer production. A combined hydrolysate prepared with sulfamic acid and featuring an initial glucose concentration of 20 g/L was used in all assays.
| Medium | NH4Cl (gL−1) |
MSG (gL−1) |
CDW (gL−1) |
P3HB (g L−1) |
P3HB (%) |
Y P/S (gPHB/gglu.cons) |
Prod vol max (gPHB L−1 h−1) |
|---|---|---|---|---|---|---|---|
| A | 1 | 20 | 8.6 | 1.8 | 21.3 | 0.12 | 0.028 |
| B | 1 | 0 | 4.7 | 2.7 | 56.7 | 0.11 | 0.047 |
| C | 1 | 5 | 7.1 | 3.7 | 52.2 | 0.15 | 0.057 |
| D | 1 | 10 | 8.7 | 3.5 | 40.8 | 0.16 | 0.056 |
| E | 0 | 10 | 8.3 | 3.4 | 41.1 | 0.16 | 0.056 |