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. 2020 Jun 1;7:100058. doi: 10.1016/j.mtbio.2020.100058

Fig. 3.

Fig. 3

Cell-instructive properties of THA-Col 1 addressing hMSC migration and cell attachment. (A) Confocal images of actin filament–stained hMSC micropellets embedded in THA-Col 1 (THA-Col 1, 25 mg/ml (1:1); Col 1, 5 mg/ml), THA (25 mg/ml), and Col 1 (5 mg/ml) at day 0, at day 3, and after 8 days of culture. Migration of hMSCs was seen in THA-Col 1 and Col 1 but not in THA. Col 1 hydrogel was shrinking over time and formed a small pellet of less than 2 mm in diameter (scale bar = 100 μm). (B) Area of cell migration and (C) migration length was higher in Col 1 than in THA-Col 1, increasing over time. Quantification of migration area and migration length. ∗∗p < 0.01, ∗∗∗p < 0.001. hMSC = human bone marrow–derived mesenchymal stromal cell; THA = tyramine derivative of hyaluronan.