FIGURE 5.

VEGF secretion is impaired in primary RPE cells expressing R345W-Fibulin-3. VEGF secretion levels were quantified by ELISA. Cell culture media were collected from the apical and basolateral sides of primary RPE cells on Transwell filters at 9 and 12 weeks post-infection. (A) Apical VEGF secretion was not significantly different in the R345W-Fibulin-3 cells compared to WT, (B) Basal VEGF secretion was significantly lower in the mutant group compared to the WT group at 9 and 12 weeks post-infection. Values are the mean ± SEM of individual wells (n = 8, **p < 0.01).