Figure 3.

Role of capsule in bile salt resistance. SGH10 and capsule mutants were plated on LB agar supplemented with increasing concentrations of (a) secondary bile salt, sodium deoxycholate (DOC) and (b) primary bile salt, sodium cholate (CHO). Experiments were repeated (n = 3), with similar results. (c) Resistance to bile salt is not affected by complementation in ∆wcaJ mutants but was restored by complementation in (d) ∆wza and (e) ∆wzy mutants. SGH10 and complemented capsule mutants were plated on LB agar supplemented with trimethoprim (TPT) and increasing concentrations of DOC