Figure 4.

Resistance to bile salt can be rescued in ∆wzy mutant through inactivation of wcaJ. SGH10 and complemented capsule mutants were plated on LB agar supplemented with trimethoprim (TPT) and increasing concentrations of DOC. (a) Bile salt resistance of the ∆wcaJ/wzy mutant compared to the single mutant (b) the ∆wcaJ/wzy mutant complemented with wcaJ or wzy. Experiments were repeated (n = 3), with similar results. (c) Propidium iodide accumulation of SGH10 and capsule mutants during sodium dodecyl sulfate (SDS) stress. Mean ± SD are plotted (n = 3) and Dunnett's multiple comparison test was conducted for a.u. values of the capsule mutants relative to SGH10 wild type at 60 min. * denotes p < .05, ** denotes p < .001, while n.s. denotes not significant [Colour figure can be viewed at wileyonlinelibrary.com]