Table 1.

List of the primers used for cloning and mutant generation

Primer name	Sequence
wcaJ Up‐for	ACATGATTACGAATTCTGCCTCTGGTAAAGGAGTGC
wcaJ Up‐rev	GCATCTTAGAAGATTTCCTTAAAATATTGAACCTT
wcaJ Dn‐for	AAATCTTCTAAGATGCTGCTTAAGATAAGCATTGT
wcaJ Dn‐rev	TTGCATGCCTGCAGAGCTCTGGCTGGTCCACTTA
wza Up‐for	ACATGATTACGAATTCTCTCCGCCAGCCAAATGAAT
wza Up‐rev	ATTTAAACATAATGTCACATCATTAGTAAACCAAGATTGC
wza Dn‐for	TGTGACATTATGTTTAAATCAGTTTTAGTTGTTTGTATTGG
wza Dn‐rev	CTTGCATGCCTGCAGTCGCCGGCATCATCAAGATT
wzy Up‐for	ACATGATTACGAATTCTGCCCTGATGCCTTTAGTTT
wzy Up‐rev	CTTTTTGCATCAAATAAAATCACACATTGCATCATTACC
wzy Dn‐for	TTTATTTGATGCAAAAAGATTTAAAATAATAGAGGAAGAACAAT
wzy Dn‐rev	CTTGCATGCCTGCAGCAACCCGGTCTGTAATGCCT
lacZ Up‐for	TATGACATGATTACGAATTCGCCATCTGATCGTTTGCCAC
lacZ Up‐rev	CCACGGATTACATATTAAACCCCGGTAAGT
lacZ Dn‐for	GTTTAATATGTAATCCGTGGGGGCGACAGC
lacZ Dn‐rev	CCAAGCTTGCATGCCTGCAGCCGAGAATACGCACCGACAT
rmpA Up‐for	CTTGCATGCCTGCAGCTTTAGTTAAGGCGGCCTTCG
rmpA Up‐rev	CTAGAGGATCCCCGGGTACCCATAGAAACAGTAACTTTGATCCATCAATATTCATCC
rmpA Dn‐for	CCCGGGGATCCTCTAGAGTCTAGGTAAAAAAGGGGAGGGGATG
rmpA Dn‐rev	ACATGATTACGAATTCTGAGCCAAATGTATGCCAAGG
wcaJ‐for	ACATGATTACGAATTCCGCATCACTTGATATGAATAATGTACT
wcaJ_rev	TTGCATGCCTGCAGGGTTGAAAACGGAGACGGTA
wza‐for	ACATGATTACGAATTCCCAGCGCAGGGATAGAAATA
wza‐rev	TTGCATGCCTGCAGCCGATCTTGAAATCTTTCAGTCTT
wzy‐for	ACATGATTACGAATTCAACTTGAACGAGCAATTCAATC
wzy‐rev	TTGCATGCCTGCAGATGGCCATTTGCGTTAGTACA