FIGURE 4.

Evoked vascular responses to 20‐second forepaw stimulation in spontaneously breathing (KX) and mechanical ventilated(MV) ketamine/xylazine‐anesthetized mice. (A, B) Averaged time courses of arterial diameter (red), venous diameter (blue) and CBVw responses (green) during 20‐second forepaw stimulation (A: n = 6 and B: n = 5 animals). The amplitude was determined as an average of 5‐20 seconds of data (marked ii) period) after the onset of stimulation for forepaw stimulation. (C) Average amplitudes of arterial dilation (red), venous dilation (blue) and CBVw OIS (green). Error bars: SEM; filled bars: spontaneously breathing ketamine/xylazine; right hatched bars: mechanically ventilated ketamine/xylazine; * P < .05. (D) Relative diameter changes in individual vessels induced by forepaw stimulation (42 vessels in KX‐SB and 37 vessels in KX‐MV)