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. 2020 Feb 3;36(3):e2966. doi: 10.1002/btpr.2966

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© 2020 The Authors. Biotechnology Progress published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fed‐batch fermentation parameters tested for P. pastoris expressing a NRRV. Proportion of full‐length P2‐VP8‐P[4] and truncated species determined by intact mass spectrometry and dry cell weight (DCW) at two different time points post‐inoculation. (a) Fermentations carried out at the indicated pH, (b) fermentations carried out with a pH pulse or pH shift, (c) scale for P2‐VP8‐P[4] concentration, the area of the circle showing full‐length and truncated P2‐VP8‐P[4] species (a and b) is proportional to its concentration in the supernatant