Figure 7.

Comparison of optimized and standard fed‐batch fermentation (USP) and purifications processes (DSP) for the production of NRRV serotype P[4]. (a) pH traces for fermentations in BSM or RDM carried out at constant pH or with a pH pulse, with its corresponding proportion of full‐length and truncated P[4] at harvest and after CIEX and AIEX; DSP pre‐treatment (C or D) and number of chromatography steps see Figure 5b. (b) Intact mass spectrometry from fermentation samples of NRRV P2‐VP8‐P[4] and P2‐VP8‐P[8] serotypes