Table 1.
Affinity resins screened for potential first bind‐elute chromatography step
| Resin class—HICa | Resin class—CIEX | Resin class—MM | |||
|---|---|---|---|---|---|
| Resin | Relative static binding capacity | Resin | Relative static binding capacity | Resin | Relative static binding capacity |
| Capto phenyl (high sub) b | 1.00 | Capto S ImpAct | 1.00 | Nuvia cPrime | 1.00 |
| Phenyl Sepharose 6 FF (low sub) | 0.64 | Capto S c | 0.90 | Capto MMC | 0.32 |
| Capto butyl | 0.93 | SP Sepharose FF | 0.40 | Capto MMC ImpRes | 0.32 |
| Phenyl Sepharose 6 FF (high sub) | 1.10 | Capto SP ImpRes | 1.00 | PPA Hypercel | 0.30 |
| Butyl‐S Sepharose 6 FF | 0.46 | Toyopearl MX‐Trp‐650 M | 0.30 | ||
| Octyl Sepharose 4 FF | 0.89 | CMM Hypercel | 0.85 | ||
| Butyl Sepharose 4 FF | 0.66 | ||||
| Capto Octyl | 0.56 | ||||
a
Resins from the following functional classes were screened; hydrophobic interaction (HIC), cation exchange (CIEX), and multimodal cation exchange (MM).
b
The best performing resin from each class is indicated in bold.
c
Resins shown italicized had adequate binding capacity but significant product truncation occurred during purification as determined by SDS‐PAGE and MS.