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. 2020 Feb 8;68(7):1445–1465. doi: 10.1002/glia.23792

Figure 6.

Figure 6

Photolytic lesions in mmp‐9 mutants results in the over production of injury‐induced progenitors and regenerated photoreceptors. (a) BrdU‐labeled cells (green) in wild‐type and mmp‐9 mi5003. (b) Number of BrdU+ cells from wild‐type (109 ± 19.66 cells; n = 6) mutant retinas (142.3 ± 25.72 cells; n = 9) at 72 hpl. *p = .0186. (c) BrdU‐labeled cells (green) in wild‐type and mutant retinas at 168 hpl. (d) Number of BrdU+ cells in the ONL of wild‐type (48 ± 10.24 cells; n = 8) and mutant retinas (71.71 ± 3.7 cells; n = 8). *p = .0001. (e) Double labeled, regenerated photoreceptors using in situ hybridization for rods (rho) and cones (pde6c; red signal) and BrdU (green) at 168hpl. (f) Number of regenerated cone photoreceptors in wild‐type (31.42 ± 9.88 cell; n = 8) and mutant retinas (43.83 ± 10.68 cells; n = 8) at 168 hpl. *p = .0301. (g) Number of regenerated rod photoreceptors in wild‐type (14.88 ± 4.02 cells; n = 8) and mutant retinas (26.04 ± 5.69 cells; n = 8) at 168 hpl. *p = .0005. Scale bars equal 50 μm. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer [Color figure can be viewed at wileyonlinelibrary.com]