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. 2020 Jun 19;11:649. doi: 10.3389/fgene.2020.00649

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Copyright © 2020 Deng, Zhang, Zhang, Huang, Li, Ni and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PCR detection of 17 putative W-linked sequences in a pair of H. armigera pupae. Genomic DNA extracted from one female (top panel) and one male (bottom panel) pupae of H. armigera were used as the templates to PCR-amplify EF-1α (as a positive control for autosome genes) and 17 selected putative W-linked sequences using the primers (Supplementary Table S1) designed based on their transcripts. The lane names starting with “SRR101” represent the PCR products of the 17 putative W-linked sequences. Std, molecular weight marker. EF-1α-NC, EF-1α negative control (no template control).