Skip to main content
. 2020 Jun 19;11:649. doi: 10.3389/fgene.2020.00649

FIGURE 2.

FIGURE 2

Sex identification of ten pairs of morphologically pre-sexed H. armigera pupae with the 206 bp GUW1 amplicon (A) and the 423 bp AF18 amplicon obtained at the annealing temperature of 62°C (B) and 58°C (C), respectively. Genomic DNA samples were individually extracted from ten pairs of morphologically pre-sexed pupae and used as the templates to PCR-amplify GUW1 with the primer pair GUW1-F and GUW1-R (Supplementary Table S2) and AF18 with the primer pair AF18-F and AF18-R (Supplementary Table S2). Lanes numbered with 1–10 indicate the 10 female (top panel) or male (bottom panel) pupae in each figure. Std, molecular weight marker. NC, negative control (no template control) for GUW1 (A) or AF18 (B–C).