FIGURE 3.

Sex identification of ten pairs of morphologically pre-sexed H. armigera pupae with the 436 bp AF18 amplicon (A) and the 118 bp GUW1 amplicon (B). Genomic DNA samples from the same ten pairs of pupae as in Figure 2 were used as the templates to PCR-amplify GUW1 with the primer pair GUW1-118-F and GUW1-118-R (Supplementary Table S2) and AF18 with AF18-436-F3 and AF18-436-R3 (Supplementary Table S2). Lanes numbered with 1–10 indicate the 10 female (top panel) or male (bottom panel) pupae in each figure. Std, molecular weight marker. NC, negative control (no template control) for AF18 (A) and GUW1 (B).