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. 2020 Jun 19;11:649. doi: 10.3389/fgene.2020.00649

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Copyright © 2020 Deng, Zhang, Zhang, Huang, Li, Ni and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sex identification of H. armigera cell line QB-Ha-E-1 with the 206 bp GUW1 amplicon (left panel) and Hadsx transcript (right panel). The gDNA samples extracted from a pair of pre-sexed pupae as well as the gDNA and total RNA samples from the H. armigera cell line QB-Ha-E-1 were used as the templates to PCR-amplify GUW1 (left panel) with the primer pair GUW1-F and GUW1-R (Supplementary Table S2) and RT-PCR-amplify Hadsx transcripts (right panel) with the primers Hadsx-F and Hadsx-R (Supplementary Table S2), respectively. Std, molecular weight marker.