Figure 4.

Effects of LY294002 exposure on GPS-treated antioxidative stress and antilipogenesis in HepG2 cells. (a) All cells were subjected to 50 μM LY294002 for 1 h; afterwards, treatment with 200 μM GPS lasts for 24 h. The expressions of PI3K, AKT, Nrf2, HO-1, NQO1, GCLM, PPARα, and SREBP-1c were assayed by Western blotting assays. (b–i) Measurement of p-AKT/AKT, p-PI3K/PI3K, Nrf2/β-actin, HO-1/β-actin, NQO1/β-actin, GCLM/β-actin, PPARα/β-actin, and SREBP-1c/β-actin was quantificated by ImageJ gel software. All data are presented as mean ± SD (three independent experiments). ^#p < 0.05 and ^##p < 0.01 vs. control group; ⁺⁺p < 0.01 vs. GPS-treated group.