Figure 2.

Oscillatory shear rheology characterization of COL1 hydrogels with varying extent of photocrosslinking and control samples. A) Measurement of storage and loss moduli for each ECM condition. Values shown are for angular frequency of 20 rad/s, obtained from full frequency sweeps (Supplementary Figure 1). Hydrogel stiffness increases up to a blue light dose of about 25 J/cm², above which photodegradation effects become significant and matrix stiffness decreases. B) COL1 yield stress as a function of crosslinking is consistent with the above data, showing increasing mechanical strength with light dose up to about 25 J/cm².