Fig. 7.

Adipose and neural cell specific staining for bioprinted MSCs in bioink with different concentration of β-GPS. a) Oil Red O staining for bioprinted MSCs in bioink with 0.6 M and 1.0 M β-GPS after 14 and 21 days of culture. The orange and red color indicates lipid droplets inside cells. (scale bar = 200 μm); b) Nissl body specific staining for bioprinted MSCs in bioink with 0.6 M and 1.0 M β-GPS after 21 days of culture. The blue color indicates large extranuclear RNA granules in neurons or neuron-like cells (scale bar = 50 μm). Quantification of c) Oil red and d) Nissl body specific staining was performed by mean grey value from three random pictures. *** indicates statistical significance (p < 0.001). NS indicates no statistical significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)