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FP binding properties of fluorescent probes to NRP1‐b1 protein
| FP | ||||
|---|---|---|---|---|
| 5‐flu probe | Probe conc (μM) | NRP‐b1 conc (μM) | Dynamic window (mP) | NRP1 b1 KD (μM)a |
| RPARPAR | 7 | 2.81b | 22.6 | 1.88 ± 1.4 |
| (3) | 1 | 10 | 30.7 | 4.61 ± 0.19 |
| (4) | 0.3 | 3 | 5.2 | 0.62 ± 0.098 |
| DS108 | 0.75 | 7.5 | 77.8 | 0.248 ± 0.097 |
Abbreviations: FP, fluorescence polarization; NRP1, neuropilin‐1.
KD derived from the 50% signal maximum.
Maximum NRP1‐b1 concentration attempted.
