Figure 2.

Expression of IL‐13 induces expression of both type‐2 and pro‐neutrophilic mediated markers. Relative mRNA expression in whole‐lung lobe lysates from littermate controls (white bars) vs Ccsp/Il13 mice (grey bars) after induction of IL‐13 for 7 d for A, Ccl11, B, Postn, C, Serpinb2, E, Cxcl1/KC, F, Cxcl2 and G, Csf3. ELISAs for D, CCL11 and H, CXCL1 protein levels in BALF. For mRNA expression, n = 22 for controls and n = 23 for Ccsp/Il13 mice and n = 7 and n = 10, respectively, for protein, from three independent experiments. Non‐parametric data are shown as box plots with medians and 25th to 75th percentiles, and whiskers representing minimum and maximum values with all data points shown; parametric data are shown as mean + SD. Statistical analyses were performed using Mann‐Whitney test or Student's t test. ***P < .001