Table 2.
NPB‐α2AP peptides identified by Mascot and MaxQuant analyses after Arg‐C digest (Figures S1‐S6)
| Sequence | Mascot analysis | MaxQuant analysis | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Amino acids | Frequency | Mass | m/z | Dev | Score | Frequency | Mass | m/z | Dev | Score | |
| R.NPNPSAPREL.K | 408‐417 | 1 | 1093.5518 | 547.7832 | 0.18 | 35 | n.d. | n.d. | n.d. | n.d. | n.d. |
| R.NPNPSAPRELKE.Q | 408‐419 | 2 | 1350.6891 | 451.2374 | 0.89 | 34 | n.d | n.d | n.d | n.d | n.d |
| R.NPNPSAPRELKEQ.Q | 408‐420 | 5 | 1478.7477 | 740.3826 | 2.03 | 58 | 1 | 1478.7478 | 740.3826 | 1.44 | 185 |
| R.NPNPSAPRELKEQ.Q | ‐ | ‐ | ‐ | ‐ | ‐ | 1 | ‐ | 494.2582 | ‐2.63 | 101 | |
| R.NPNPSAPRELKEQQ.D | 408‐421 | 53 | 1606.8063 | 804.4133 | 3.62 | 71 | 25 | 1606.8063 | 536.60947 | ‐2.14 | 193 |
| R.NPNPSAPRELKEQQ.D | 49 | ‐ | 536.6099 | 0.91 | 55 | ‐ | ‐ | ‐ | ‐ | ‐ | |
| R.NPNPSAPRELKEQQ.Da | 408‐421 | 24 | 1607.7903 | 804.9037 | 1.57 | 68 | 10 | 1607.7903 | 804.9047 | 2.21 | 234 |
| R.NPNPSAPRELKEQQ.Da | 19 | ‐ | 536.9383 | 1.71 | 49 | 15 | ‐ | 536.94424 | 1.96 | 187 | |
| R.NPNPSAPRELKEQQD.S | 408‐422 | 1 | 1721.8332 | 861.9233 | ‐0.71 | 33 | 1 | 1721.8333 | 861.9233 | 0.19 | 163 |
| R.NPNPSAPRELKEQQD.S | 8 | ‐ | 574.9523 | 1.01 | 61 | 6 | ‐ | 574.9523 | ‐1.27 | 213 | |
Dev, the difference between the observed and calculated mass (in ppm); frequency, the number of times a peptide is observed in the mass spectrometry analysis; mass: the theoretical monoisotopic mass of the neutral peptide (in Daltons); m/z, the observed mass/charge; n.d., not determined; score, the ions score given to the peptide.
Best scoring spectra are reported.
a
Deamidation.