Table 1.

Predicted small secreted Plasmodiophora brassicae proteins (SSPbPs) with SP score > 0.7 and length < 400 aa

SSPbP no.	Length (aa)	MW (kDa)	IPa	SPb score	Cleavage site (bp)	Cleavagec score	ID and/or motifs	Localizationd	Plasmodiophora brassicae (Pb) protein
SSPbP01	213	23.86	5.9	0.857	26	0.710	HPe	C	PBRA_000804
SSPbP02	398	43.9	5.79	0.827	22	0.723	Glucanase	N	PBRA_002335
SSPbP03	390	41.87	10.77	0.866	20	0.824	Serine protease	Ch, M	PBRA_002618
SSPbP04	123	12.95	9.49	0.869	28	0.682	Cons. HPf	N	PBRA_005973
SSPbP05	335	35.68	9.56	0.824	23	0.521	SMCg	C	PBRA_002298
SSPbP10	368	38.54	5.34	0.854	22	0.650	Cons. HP	C	PBRA_007831
SSPbP11	301	33.42	5.62	0.773	23	0.378	HP	C	PBRA_004359
SSPbP18	287	41.03	6.95	0.906	22	0.811	HP	C	PBRA_001856
SSPbP21	283	30.48	6.4	0.759	26	0.433	SMC	C	PBRA_002100
SSPbP22	375	41.95	5.21	0.778	28	0.567	Kinase	C, N	PBRA_008980
SSPbP31	266	27.73	7.02	0.727	18	0.351	HP	N	PBRA_005440
SSPbP32	204	22.01	6.85	0.779	30	0.632	Cons. HP	A, Ch	PBRA_008250
SSPbP41	386	40.00	4.99	0.906	24	0.913	HP	A	PBRA_006112
SSPbP42	206	21.51	7.81	0.751	18	0.438	Cons. HP	N	PBRA_005499
SSPbP43	311	33.68	5.02	0.860	26	0.747	ANKh	C	PBRA_006649
SSPbP44	96	9.65	5.23	0.798	21	0.566	HP	A	PBRA_005716
SSPbP51	395	42.34	7.92	0.916	22	0.832	Cons. HP	Ch, M, N	PBRA_007344
SSPbP52	206	28.39	4.96	0.840	17	0.536	HP	N	PBRA_002122
SSPbP53	140	14.55	4.55	0.819	24	0.596	Cysteine protease inhibitor	A	PBRA_008207
SSPbP54	248	27.4	8.54	0.776	20	0.792	Cons. HP	M	PBRA_003240
SSPbP61	366	40.06	7.31	0.755	25	0.318	Kinase	N	PBRA_001067
SSPbP62	242	26.55	4.96	0.751	22	0.372	Cons. HP	C	PBRA_009168
SSPbP71	389	42.44	7.02	0.776	31	0.649	ANK	C	PBRA_001722
SSPbP72	229	25.45	6.97	0.789	20	0.454	Cons. HP	C	PBRA_008279
SSPbP73	236	24.47	8.76	0.910	19	0.840	Cons. HP	N	PBRA_001286
SSPbP81	200	21.98	5.07	0.765	18	0.558	Cyclin	C	PBRA_005813
SSPbP86	227	25.47	6.12	0.871	23	0.622	HP	C	PBRA_000257
SSPbP91	154	15.68	9.17	0.789	20	0.789	Cons. HP	A	PBRA_005502
SSPbP92	385	41.71	8.96	0.746	26	0.362	SMC	N	PBRA_002300
SSPbP93	315	34.39	5.17	0.848	26	0.701	ANK	C	PBRA_006655
SSPbP94	307	33.32	8.46	0.745	21	0.260	Translocase	N	PBRA_005298
SSPbP97	233	25.23	5.76	0.832	20	0.622	Cons. HP	C	PBRA_003620
PbBSMTi	377	45.21	4.92	0.728	21	0.505	Methyl transferase	C	AFK13134

^aIsoelectric point of the SSPbP.

^bSignal peptide score, also known as D (discrimination) score, is the value used to distinguish proteins with signal peptides from proteins with no signal peptides.

^cCleavage score, or C‐score (cleavage), is the value that is used to determine whether a signal peptide is processed by the host cell and removed in the mature protein.

^dA = apoplast; C = cytoplasm; Ch = chloroplast; M = mitochondria; N = nucleus.

^eHypothetical protein.

^fConserved hypothetical protein.

^gChromosome segregation protein.

^hAnkyrin domains.

ⁱMethyl transferase characterized by Ludwig‐Müller et al. (2015).