Table 1.
Animal allocation and usage
| Experiments | Groups | PCR | mNSS | WB | BWC | EB |
Cell culture |
Death | Mortality |
|---|---|---|---|---|---|---|---|---|---|
| Experiment 1 | Sham | 5 | – | – | – | – | – | 0 | 0 |
| CCI | 8 × 4 | – | – | – | – | – | 4 | 12.5% | |
| CCI + antagomir | 8 × 4 | – | – | – | – | – | 6 | 18.75% | |
| CCI + agomir | 8 × 4 | – | – | – | – | – | 6 | 18.75% | |
| Experiment 2 | Sham | – | 8 | – | – | – | – | 0 | 0 |
| CCI | – | 8 | – | – | – | – | 2 | 25% | |
| CCI + antagomir | – | 8 | – | – | – | – | 3 | 37.5% | |
| CCI + agomir | – | 8 | – | – | – | – | 1 | 12.5% | |
| Experiment 3 | Sham | – | – | 6 | 6 | 6 | – | 0 | 0 |
| CCI | – | – | 6 | 6 | 6 | – | 1 | 5.56% | |
| CCI + antagomir | – | – | 6 | 6 | 6 | – | 2 | 11.11% | |
| CCI + agomir | – | – | 6 | 6 | 6 | – | 2 | 11.11% | |
| Experiment 4–6 | BMEC/Astrocyte | – | – | – | – | – | 10 | – | – |
| Total number | Adult: 205 | 101 | 32 | 24 | 24 | 24 | – | 27 | 13.17% |
| immature:10 | – | – | – | – | – | 10 | – | – |
Detailed information about animal allocation and usage in this study. Although compared with the rats which suffered control cortex impact, the mortality number in each subexperiment and overall mortality number were higher in CCI + antagomir group, no significant difference was achieved.
Abbreviations: CCI, control cortex impact; WB, western blot; BWC, brain water content; EB, Evans Blue; BMEC, brain microvascular endothelial cell.