FIG 6.

Scheme illustrating the treatment regimen of dogs naturally infected with L. infantum. Dogs were treated with mixed or conventional liposome formulation of MA plus allopurinol or allopurinol alone. Liposome formulations (LCP, mixed-liposome formulation; LC, conventional liposome formulation) were given intravenously as two cycles of six doses (6.5 mg Sb/kg/dose) at 4-day intervals. Allopurinol (Allop) was given at 30 mg/kg/12 h per os for 130 days, starting 30 days before the first dose of liposomal MA. The samples of tissues and blood were collected from all animals and clinically evaluated in times before, just after, and 4 months after treatment protocols. No intervention was performed on animals for 4 months after treatment for assessment of relapses.