FIG 4.

Nitrite level in response to treatment with benznidazole (BZ) or combined treatment with aspirin (ASA) in mice infected with T. cruzi. The antagonist Boc-2 (10 μM/kg) was administered via intraperitoneal injection from 1 day postinfection every 48 h for 7 days before ASA treatment. After 180 days postinjection, the mice were euthanized. Uninfected control (NI) mice were also evaluated. NO was determined by measuring nitrite levels in plasma (A) and cardiac tissue (B) using the cadmium-copper system followed by the Griess reaction. The results are expressed as means ± standard errors of the means from 5 animals per group and are representative of 2 independent experiments. Means not sharing a letter are significantly different (P < 0.05; 2-way ANOVA with Tukey posttest).