FIG 1.

Inhibition of VP24 by gossypetin, taxifolin, and tricetin. (A) Chemical structures of gossypetin, taxifolin, and tricetin. (B) HEK293T cells were transfected with pISRE-luc, Renilla luciferase-thymidine kinase DNA (RL-TK), and pcDNA3.1 or VP24 expression plasmid. Twenty-four hours after transfection, cells were stimulated with IFN-α, and gossypetin, taxifolin, and tricetin were added. Then luciferase activity was measured. Results are shown as percent ISRE expression in VP24-transfected cells over empty vector control. Firefly luciferase activity was normalized to the Renilla luciferase internal control. Data represent the means ± SDs from at least three independent experiments. Asterisks indicate significant difference (two-tailed unpaired Student’s t test, n = 3). *, P < 0.05; **, P < 0.01.