FIG 2.

Effects of screened flavonoids on EBOV VP24 inhibition of IFN signaling. (A) Chemical structures of quercetin and wogonin. (B) HEK293T cells were transfected with pISRE-luc, RL-TK, and pcDNA3.1 or EBOV VP24 expression plasmid. After 24 h, cells were treated with IFN-α and compounds. After 8 h of stimulation, luciferase activity was measured. Results are shown as percent ISRE expression in VP24-transfected cells over pcDNA3.1 control. (C and D) After 24 h of cotransfection with pISRE-luc, RL-TK, and pcDNA3.1, HEK293T cells were stimulated with IFN-α and compounds. Results are shown as percent ISRE expression in compound-treated cells over untreated control. Firefly luciferase activity was normalized to the Renilla luciferase internal control. Data represent the means ± SDs from at least three independent experiments. Asterisks indicate significant difference (two-tailed unpaired Student’s t test, n = 3). *, P < 0.05; ***, P < 0.005.