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. 2020 Jun 23;64(7):e00530-20. doi: 10.1128/AAC.00530-20

FIG 4.

FIG 4

Effect of quercetin on VP24 inhibition of P-STAT1 nuclear translocation. (A) Rows 1 and 2, immunofluorescence of HEK293T cells cotransfected with empty vector (EV) not stimulated (row 1) or stimulated (row 2) with IFN-α for 30 min. Rows 3 and 4, immunofluorescence of HEK293T cells cotransfected with FLAG-tagged VP24 plasmid stimulated with IFN-α and not treated (row 3) or treated (row 4) with quercetin (30 μM). Asterisk indicates P-STAT1 in the nucleus of a VP24 cell treated with quercetin. All cells were stained for FLAG and P-STAT1. Scale bar, 10 μm. (B) Percentage of nuclear P-STAT1 within transfected cells expressing VP24. Data represent the means ± SDs from three independent experiments. ****, P < 0.0001, two-tailed unpaired Student’s t test (n = 3).