FIG 5.

(A) Three-dimensional representation of the putative binding mode obtained by blind docking of quercetin into VP24. (B) Relative 2D representation of the complexes stabilizing interactions. (C) Alignment of quercetin-VP24 complex obtained by docking experiment with VP24-KPNα5 reported in the model with PDB code 4U2X (9). (D) Schematic representation of KPNα-VP24 co-IP experiment in the presence of quercetin. (E) HEK293T cells were cotransfected with a V5-tagged form of EBOV VP24 and FLAG-tagged KPNα1. Empty vector was used as a negative control. At 24 h posttransfection, cells were lysed and co-IP was performed using anti-FLAG magnetic beads (IP:FLAG). Immunoprecipitated proteins were detected by Western blotting with antibodies to V5 (WB:V5) and FLAG (WB:FLAG). The presence of transfected proteins in whole-cell extracts (WCE) was verified by detection with the corresponding antibodies. Band quantification was performed.