Figure 2.

Fabrication of glomerulus-on-a-plate platform. (A–C) The generation of the microconvex topography on the hydrogel knot surface using chemically induced inflation method. (A) Knotted hydrogel microfiber with embedded CaCO₃ beads. (D) Fluorescent image of the h-FIBER (TRITC-conjugated fluorescent beads were embedded in the hydrogel). (E) Low-magnification and (F) high-magnification SEM image showing the microconvex topography on the scaffold surface. (G) Assembly of the h-FIBERs into a 96-well plate. (H) Gravity-driven perfusion of the h-FIBER. (I) Perfusion of the h-FIBER by the syringe pump. (J) Schematics of the syringe pump connection. (K) Pump-driven flow at 70 μL/min (blue food dye indicator). Scale bars: 200 μm.