Skip to main content
. 2020 Jun 19;11:1254. doi: 10.3389/fimmu.2020.01254

Figure 2.

Figure 2

Evaluation of the effect of Adjuvants on FDC Networks. (A) Representative images for FDC expression in draining lymph nodes 24 h post subcutaneous injections of either OVA-A594, OVA-A594 + PorB, OVA-A594 + CpG, or OVA-A594 + Alum. FDC expression is shown as a heat map where white indicates the highest signal to pixel ratio and blue sows the lowest signal to pixel ratio. Scale bar is 100 μM. One of 3 representative experiments is shown. (B) Mean fluorescence intensity (MFI) quantification from ImageJ of FDC networks in draining lymph nodes 24 h post injection of either OVA-A594, OVA-A594 + PorB, OVA-A594 + CpG, or OVA-A594 + Alum. Multiple FDC networks were measured within individual lymph nodes. n = 9/group. *p < 0.05, ***p < 0.001 (C) Frequency of FDC in draining lymph nodes 24 h post subcutaneous injections with OVA-A594 ± adjuvants. Gating strategy is shown in Supplemental Figure 4. (D) Cell counts of FDC in draining lymph nodes 24 h post subcutaneous injections with OVA-A594 ± adjuvants. (E) MFI of intercellular adhesion molecule 1 (ICAM-1) from FDC gate in draining lymph nodes 24 h post subcutaneous injections with OVA-A594 ± adjuvants. (F) MFI of complement receptors 1 and 2 (CR1/2) from FDC gate in draining lymph nodes 24 h post subcutaneous injections with OVA-A594 ± adjuvants. n = 9 per group *p < 0.05, **p < 0.01; ns, not significant.